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The process that destroys or removes all microorganisms and microbial forms, including bacterial endospores, on inanimate objects is ______.


A) disinfection
B) sterilization
C) antisepsis
D) sanitization
E) degermation

F) D) and E)
G) A) and E)

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Soaps and detergents are very effective as sterilants.

A) True
B) False

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The presence of organic matter such as saliva and pus can interfere with the actions of disinfectants.

A) True
B) False

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Which of the following is an advantage to using phenolics as antimicrobial agents?


A) The presence of organic matter does not limit its antimicrobial qualities.
B) They are not susceptible to resistance by organisms.
C) They have low toxicity.
D) They are effective sterilants against all microbial forms.

E) None of the above
F) B) and C)

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Isopropyl alcohol wiped across a skin site can sterilize it.

A) True
B) False

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The easiest microbial forms to kill or inhibit are ______.


A) naked viruses
B) vegetative bacteria and fungi
C) endospores
D) protozoan cysts
E) mycobacteria and staphylococci

F) B) and C)
G) A) and E)

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The use of chemical agents directly on exposed body surfaces to destroy or inhibit vegetative pathogens is ______.


A) disinfection
B) sterilization
C) antisepsis
D) sanitization

E) None of the above
F) A) and B)

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Which is correct regarding the rate of microbial death?


A) Cells die at increasingly greater rates.
B) Only older cells die in a culture.
C) Cells in a culture die at a constant rate.
D) Upon contact with the control agent, all cells die at one time.
E) Cells become metabolically inactive but are never killed.

F) None of the above
G) C) and D)

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Your aim is to sterilize prosthetic devices like heart valves and artificial joint structures before being used in the patient.Considering where they will be placed and the probable composition of the devices, what would be the best chemical to use?


A) Crystal violet dye
B) Iodine
C) Quaternary ammonium compounds
D) Bleach

E) C) and D)
F) B) and D)

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Antimicrobial agents can target the cell wall by


A) blocking its synthesis.
B) digesting it.
C) denaturing proteins.
D) All of the choices are correct.

E) A) and B)
F) A) and C)

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You have some old plastic Petri dishes that you would like to use for pouring bacteriological agar plates.The only method of physical sterilization is ultraviolet radiation sterilization with your UV light (you cannot use the autoclave because it will disfigure and melt the plastic) .After sterilizing the plates, pouring the sterilized agar medium, and then leaving the plates out at room temperature for a day or two to let them solidify and dry, you find contaminating bacterial colonies growing on the agar.Predict what has happened in this situation.


A) The room temperature enhanced the growth of normal biota of the agar.
B) Air got into the poured agar plates, contaminating them.
C) The lids of the Petri dishes were inadvertently left on while being sterilized.
D) The agar had bacteria in it, even after sterilization in the autoclave.

E) B) and C)
F) None of the above

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Prions require more extensive methods of sterilization than are needed for bacterial endospores.

A) True
B) False

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Which of the following is not a primary target of milk pasteurization?


A) Salmonella
B) Campylobacter jejuni
C) Lactobacillus
D) Listeria monocytogenes
E) Brucella

F) B) and D)
G) C) and D)

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The lowest temperature needed to kill or inactivate all microbes in 10 minutes is the


A) thermal death point (TDP) .
B) thermal death time (TDT) .
C) sporicidal time.
D) death phase point.

E) A) and B)
F) A) and C)

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Each of the following is a target of antimicrobial agents except ______.


A) cell walls
B) cell membranes
C) ribosomes
D) cellular proteins
E) cytoplasm

F) B) and D)
G) A) and B)

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What instrument is most effective for pressure-temperature sterilization?


A) Water-bath
B) Autoclave
C) Incubator
D) Oven
E) Bunsen burner

F) C) and E)
G) B) and E)

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Endospores can be inactivated by


A) dry heat at 170°C for 2 hours.
B) incineration.
C) glutaraldehyde for 3 or more hours.
D) ethylene oxide for 3 or more hours.
E) All of the choices are correct.

F) None of the above
G) B) and C)

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Agents that can denature microbial proteins include all of the following except ______.


A) moist heat
B) alcohol
C) acids
D) metallic ions
E) X rays

F) B) and D)
G) B) and C)

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All of the following are alkylating control agents except ______.


A) iodophor
B) propylene oxide
C) formaldehyde
D) ethylene oxide
E) glutaraldehyde

F) A) and E)
G) B) and E)

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Cold temperatures are considered microbistatic, whereas excessive heat is considered microbicidal. This is due to the fact that


A) in cold temperatures, the energy of activation is not sufficient to drive chemical reactions regardless of the

presence of enzymes, whereas excessive heat denatures enzymes and/or incinerates the cell causing irreparable damage.

B) cold temperatures denature enzymes causing irreparable damage to the cell, whereas heat limits the energy of activation available to drive chemical reactions.

C) cold temperatures freeze and therefore kill the cell, whereas excess heat keeps the cell metabolically active but not dividing.

D) cold temperatures halt binary fission and is therefore considered a sterilant, whereas heat does not kill spores so . it is not an effective method of control

E) None of the above
F) A) and B)

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